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Specialty Chicken Bone Peptone - Optimized Nitrogen Substrate for Advanced Fermentation with Balanced Peptide Profile

Basic Information
Place of Origin: CHINA
Brand Name: SHIHONG
Certification: SGS
Model Number: PEPTONE
Minimum Order Quantity: 1MT
Price: USD 2.3/KG - USD 3.1$/KG
Packaging Details: 20KG/BAG 25KG/BAG
Delivery Time: 5 WORKING DAYS
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 2,000MT/MONTH
Detail Information
Highlight:

Optimized Nitrogen Substrate Peptone

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Advanced Fermentation Processes Chicken Bone Peptone

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Balanced Peptide Profile Nitrogen Substrate


Product Description

Specialty Chicken Bone Peptone — Optimized Nitrogen Substrate for Advanced Fermentation Processes

Product Description

Specialty Chicken Bone Peptone is a scientifically formulated nitrogen source produced from fresh poultry bone stock through a multi-stage enzymatic hydrolysis process. This product delivers a balanced peptide profile specifically optimized for demanding microbial fermentation systems, vaccine production platforms, and specialized culture media formulations. Its consistent nutrient composition supports reproducible biomass accumulation and metabolite production across various bacterial and fungal strains.


Raw Material Profile

 
 
Attribute Specification
Base Material Fresh chicken bone from USDA-inspected facilities
Collection Protocol Temperature-controlled processing within 4 hours of slaughter
Quality Criteria Free from pathological tissue, chemical residues, and environmental contaminants
Supply Chain Documented traceability from source to finished product

Manufacturing Workflow

 
 
Processing Module Technical Parameters
Initial Processing Cryogenic grinding, thermal sanitation, fat separation
Hydrolysis System Sequential enzyme treatment with endo- and exopeptidases
Clarification Train Depth filtration → activated carbon treatment → membrane filtration (0.45μm)
Evaporation Stage Falling film evaporation at ≤60°C
Drying Technology Multi-stage spray drying with particle size classification
Quality Verification Real-time NIR monitoring + comprehensive batch testing

Functional Attributes

  • Peptide Distribution – Optimized molecular weight profile (500-3000 Da) enhances microbial uptake efficiency

  • Mineral Balance – Controlled cation/anion ratio minimizes precipitation in media formulations

  • Thermal Stability – Maintains solubility and nutrient availability through standard sterilization cycles

  • Lot Uniformity – Statistical process control ensures CV <5% for key parameters across production batches

  • Endotoxin Control – Low endotoxin levels suitable for sensitive biological applications


Analytical Specifications

 
 
Parameter Test Method Unit Specification Range Lot Analysis
Total Organic Nitrogen Kjeldahl (GB 5009.5) % 14.0 – 15.5 14.8
Primary Amino Groups Formol titration % ≥ 2.5 2.9
Chloride (as NaCl) Argentometric (GB/T 12457) % ≤ 5.0 2.3
Residual Moisture Gravimetric (GB 5009.3) % ≤ 6.0 4.2
Incineration Residue 550°C (GB 5009.4) % ≤ 6.0 4.5
Aqueous pH Potentiometric (2% solution) pH units 5.2 – 6.8 5.6
Optical Density (620nm, 2% soln) Spectrophotometric Absorbance ≤ 0.10 0.06
Nitrite Content Diazotization (GB 5009.33) mg/kg ≤ 2.0 < 1.0
Total Heavy Metals ICP-MS mg/kg ≤ 10 < 10
Bulk Density Tapped density g/mL 0.45 – 0.65 0.52

Strain-Specific Performance Characteristics

 
 
Microorganism Test Protocol Performance Indicator Result
Salmonella enterica serovar Typhi H₂S production (lead acetate method) Positive control response + (48h)
Shigella dysenteriae H₂S production (lead acetate method) Negative control response - (48h)
Escherichia coli (ATCC 25922) Indole production (Kovac's reagent) Positive response + (24h)
Salmonella enterica serovar Typhi Indole production (Kovac's reagent) Negative response - (24h)
Enterobacter cloacae Voges-Proskauer reaction Acetoin production + (24h)
Escherichia coli (ATCC 25922) Voges-Proskauer reaction Acetoin production - (24h)
Streptococcus pneumoniae Blood agar hemolysis Alpha pattern (green) Stable through 3 passages
Streptococcus pyogenes Blood agar hemolysis Beta pattern (clear) Stable through 3 passages
Pseudomonas aeruginosa Pigment expression (King A medium) Pyocyanin production Green pigment stable
Staphylococcus aureus Pigment expression (nutrient agar) Staphyloxanthin production Golden pigment stable
Escherichia coli Colony morphology (2% peptone agar) Diameter, colony type ≥1.2mm, 98% smooth
Staphylococcus aureus Colony morphology (2% peptone agar) Diameter, colony type ≥1.0mm, 97% smooth
Streptococcus pyogenes Growth sensitivity (dilution series) Maximum supporting dilution 10⁻⁴
Bacillus subtilis Growth sensitivity (dilution series) Maximum supporting dilution 10⁻⁵

Application-Specific Recommendations

 
 
Application Field Recommended Concentration Performance Benefit
Antibiotic Fermentation 1.5 – 2.5% (w/v) Enhanced secondary metabolite production
Enzyme Production 2.0 – 3.0% (w/v) Balanced C:N ratio supports protease/amylase yield
Vaccine Manufacturing 1.0 – 2.0% (w/v) Consistent antigen expression profiles
Diagnostic Media 1.0 – 1.5% (w/v) Clear background, no interfering reactions
Probiotic Cultivation 1.5 – 2.0% (w/v) High viable cell recovery
Research Media Variable Reproducible growth curves across experiments

Packaging Specifications

 
 
Component Specification
Primary Container Food-grade polyethylene liner, heat-sealed
Secondary Packaging Multilayer kraft paper bag with moisture barrier
Unit Size 20 kg net weight (±0.1 kg)
Pallet Configuration 40 bags per pallet (800 kg), stretch-wrapped
Labeling Product name, lot number, net weight, manufacture date, storage conditions

Storage and Stability

  • Optimal Storage: 15 – 25°C, ≤50% relative humidity

  • Shelf Life: 24 months from manufacture date

  • Stability Indicators: Retain sealed until use; protect from moisture, heat, and direct light

  • Post-Opening Handling: Use within 30 days after opening; reseal tightly after each use


Regulatory and Quality Framework

 
 
Aspect Details
Manufacturing Standard ISO 9001:2015 certified facility
Product Specification Q/SCSH 003-2025 (Chicken Peptone, Biological Grade)
Reference Documents Chinese Biological Products Raw Material Standards (2000)
Analytical Methods GB, ChP, and EP referenced procedures
Documentation Full batch traceability, COA available for each lot

For technical consultation, sample requests, or customized formulations, please contact our biotechnology support team.

Contact Details
John

Phone Number : +86 138 8078 3260

WhatsApp : +8613880783260